Whole Genome Sequencing

Based on Fluorescence Microscopic Methods

Fast, Reliable, and Low Sequencing Costs

Who we are and what we look like, which foods we can tolerate and which we cannot, the diseases that threaten an individual... that and much more is largely set in our genes. The holistic analysis of genomes is therefore becoming increasingly important. In particular, the method known as "sequencing-by-synthesis" has made enormous progress since its development. Speed and precision have increased by several orders of magnitude, while costs have also decreased by several orders of magnitude. The positioning techniques employed in the microscope systems used for this purpose have also contributed significantly to this.

DNA Recombination: Detection of Fluorescence Signals

In this method, fluorescence-labelled nucleotides are added as a complement to template DNA. Recombination produces characteristic fluorescence light signals, which are detected microscopically. In order to read out these short-lived and weak fluorescence signals reliably and comprehensibly, PI offers high-precision microscope stages for XY(Z) positioning in the Flowcell and PIFOC® objective positioning stages for the correct focus height in Z. Several drive technologies are available for each of these subsystems, each with its own characteristic properties and specifications: From piezo motors, to magnetic direct drives, and voice coil motors.

PIFOC® Objective Scanner

High position resolution in the nanometer range
Fast positioning
Large range of products and properties
Travel ranges of a few µm to 2 mm

XY(Z) Sample Stage

Wide variety of products and properties enables customer-specific adaptation
Velocities to 500 mm/s
High position resolution in the nanometer range
Travel ranges to 135 x 85 mm
  • Settling time < 20 ms
  • Travel ranges from 100 – 460 µm (delivery without objective)
  • Velocity: to 200 mm/s
  • Travel ranges: 205 x 205 mm
  • Unidirectional repeatability to 0.1 µm
  • Settling time < 5 ms
  • Travel range 18 µm
  • 2-phase stepper or DC motors
  • Velocity: 90 mm/s
  • Travel ranges (X - Y): 205 mm x 205 mm
  • Unidirectional repeatability to 0.05 µm
  • Settling time 6 ms
  • Travel range 100 µm
  • Velocity: 120 mm/s
  • Travel ranges 100 – 135 mm x 75 – 85 mm

Voice Coil PIFOC®

 

  • Long strokes of several mm
  • Velocity > 250 mm/s; travel range > 5 nm
  • High stiffness due to crossed roller bearings
  • High dynamics
  • Travel ranges (X - Y - Z): 200 µm x 200 µm x 200 µm
  • Position resolution: 1 nm